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Image Search Results
Journal: Frontiers in Bioengineering and Biotechnology
Article Title: Combining photodynamic therapy and cascade chemotherapy for enhanced tumor cytotoxicity: the role of CTT 2 P@B nanoparticles
doi: 10.3389/fbioe.2024.1361966
Figure Lengend Snippet: ROS generation detection in vitro . (A) The release of singlet oxygen from each drug was detected in vitro under dark conditions. Data were presented as the mean ± SD ( n = 3). (B) Detection of singlet oxygen release of each drug under 660 nm laser irradiation at a power density of 280 mW cm -2 was performed in vitro . Data were presented as the mean ± SD ( n = 3). (C) ROS generation was observed in 4T1 cells treated with each medication while being exposed to laser irradiation at a wavelength of 660 nm and intensity of 280 mW cm −2 . Bright: Bright field. DCF: Green fluorescence represents intracellular ROS. Merge: Superimpose the image. Scale Bar = 100 μm.
Article Snippet: The
Techniques: In Vitro, Irradiation, Fluorescence
Journal: Frontiers in Bioengineering and Biotechnology
Article Title: Combining photodynamic therapy and cascade chemotherapy for enhanced tumor cytotoxicity: the role of CTT 2 P@B nanoparticles
doi: 10.3389/fbioe.2024.1361966
Figure Lengend Snippet: Evaluation of mitochondrial targeting function. (A) Confocal images of the mitochondrial sites of TPPOH 2 , CTT 2 , CTT 2 P, and CTT 2 P@B NPs in 4T1 cells. Mito-Tracker Green was used to stain the mitochondria in the green channel. The red channel was derived from the emission of the photosensitizer fraction (PS) itself. Merge stands for superimposed image. The Green and red curves in the Plot Profile represent the gray value of Mito-Tracker Green and PS, respectively. Scale bar = 20 μm. (B) Flow cytometry JC-1 method was used to analyze the mitochondrial function of cells treated with different drugs. Red fluorescence: normal mitochondria (J-aggregate); Green fluorescence: depolarized mitochondria (J-monomer).
Article Snippet: The
Techniques: Staining, Derivative Assay, Flow Cytometry, Fluorescence
Journal: Frontiers in Bioengineering and Biotechnology
Article Title: Combining photodynamic therapy and cascade chemotherapy for enhanced tumor cytotoxicity: the role of CTT 2 P@B nanoparticles
doi: 10.3389/fbioe.2024.1361966
Figure Lengend Snippet: Evaluation of cell death in vitro through cytotoxicity assessment and examination of apoptosis and necrosis. (A) The in vitro cytotoxicity of 4T1 cells treated with CPT, TPPOH 2 , CTT 2 , CTT 2 P, and CTT 2 P@B NPs in the dark was assessed using the CCK-8 assay. Data were presented as the mean ± SD ( n = 5). ** p < 0.01, **** p < 0.0001. (B) The in vitro cytotoxicity of 4T1 cells treated with TPPOH 2 , CTT 2 , CTT 2 P and CTT 2 P@B NPs under laser irradiation (660 nm, 280 mW cm −2 ) was determined using the CCK-8 assay. Data were presented as the mean ± SD ( n = 5). ** p < 0.01, **** p < 0.0001. (C) Cell apoptosis and necrosis were analyzed using flow cytometry with Annexin V-FITC/PI double staining following treatment with various drugs at a concentration of 5 μM.
Article Snippet: The
Techniques: In Vitro, CCK-8 Assay, Irradiation, Flow Cytometry, Double Staining, Concentration Assay
Journal: Frontiers in Bioengineering and Biotechnology
Article Title: Combining photodynamic therapy and cascade chemotherapy for enhanced tumor cytotoxicity: the role of CTT 2 P@B nanoparticles
doi: 10.3389/fbioe.2024.1361966
Figure Lengend Snippet: Biodistribution in vivo . (A) Blood compatibility test of CPT, TPPOH 2 , CTT 2 , CTT 2 P, CTT 2 P@B NPs. Data were presented as the mean ± SD ( n = 3). *** p < 0.001, **** p < 0.0001. (B) Time-lapse live fluorescence imaging of mice with 4T1 tumors following the administration of free CTT 2 P and CTT 2 P@B NPs via intravenous injection. (C) Fluorescent images of major organs and tumors were obtained 24 h after injection, using ex vivo methods. (D) The mean fluorescence intensity of each organ and tumor was used to determine the biodistribution of free CTT 2 P and CTT 2 P@B NPs in mice. Data were presented as the mean ± SD ( n = 3). * p < 0.05.
Article Snippet: The
Techniques: In Vivo, Fluorescence, Imaging, Injection, Ex Vivo
Journal: Frontiers in Bioengineering and Biotechnology
Article Title: Combining photodynamic therapy and cascade chemotherapy for enhanced tumor cytotoxicity: the role of CTT 2 P@B nanoparticles
doi: 10.3389/fbioe.2024.1361966
Figure Lengend Snippet: In vivo anti-tumor study of each drug in 4T1 tumor-bearing mice. (A) Tumor images of different drug administration treatments after the antitumor study. (B) During the administration, the growth of tumors in mice was observed in each group receiving treatment. Data were presented as the mean ± SD ( n = 5), ** p < 0.01. (C) The weight of mice in each treatment group was monitored throughout the administration period. Data were presented as the mean ± SD ( n = 5).
Article Snippet: The
Techniques: In Vivo
Journal: Frontiers in Bioengineering and Biotechnology
Article Title: Combining photodynamic therapy and cascade chemotherapy for enhanced tumor cytotoxicity: the role of CTT 2 P@B nanoparticles
doi: 10.3389/fbioe.2024.1361966
Figure Lengend Snippet: Investigation of the effects of each medication on mice with 4T1 tumors through pathological examination. (A) After administering various medications, the major organs (including the heart, liver, spleen, lung, and kidney) were subjected to H&E staining. Scale bar = 50 μm. (B) After administering various medications, tumors were subjected to H&E staining and TUNEL staining. Scale bar = 50 μm.
Article Snippet: The
Techniques: Medications, Staining, TUNEL Assay